| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Hydrolysis of galacto-oligosaccharides in soy molasses by α -galactosidases and invertase from Aspergillus terreus
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| Autores: |
Reis,Angélica Pataro
Guimarães,Valéria Monteze
Ferreira,Joana Gasperazzo
Queiroz,José Humberto de
Oliveira,Maria Goreti Almeida
Falkoski,Daniel Luciano
Almeida,Maíra Nicolau de
Rezende,Sebastião Tavares de
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| Data: |
2010-06-01
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| Ano: |
2010
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| Palavras-chave: |
Aspergillus terreus
Galactooligosaccharides
Α-galactosidase
Invertase
Soy molasses
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| Resumo: |
Two α -galactosidase (P1 and P2) and one invertase present in the culture of Aspergillus terreus grown on wheat straw for 168 h at 28ºC were partially purified by gel filtration and hydrophobic interaction chromatographies. Optimum pH and temperatures for P1, P2 and invertase preparations were 4.5-5.0, 5.5 and 4.0 and 60, 55 and 65ºC, respectively. The K M app for Ï -nitrophenyl-α -D-galactopyranoside were 1.32 mM and 0.72 mM for P1 and P2, respectively, while the K M app value for invertase, using sacarose as a substrate was 15.66 mM. Enzyme preparations P1 and P2 maintained their activities after pre-incubation for 3 h at 50ºC and invertase maintained about 90% after 6 h at 55 ºC. P1 and P2 presented different inhibition sensitivities by Ag+, D-galactose, and SDS. All enzyme preparations hydrolyzed galacto-ologosaccharides present in soymolasses.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300029
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132010000300029
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.53 n.3 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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